Fluorescent nanoparticles (FNPs) have attracted considerable interest for their wide range of emerging applications in live cell imaging, biosensing, and optoelectronic devices. Among currently available FNPs, dye-doped polymer nanoparticles are limited by fluorophore aggregation and self-quenching as well as dye leaching.
A relatively unexplored alternative is the use of FNPs with covalently bound fluorophores. Covalent attachment limits chromophore mobility and disperses the monomers within FNPs which potentially reduces fluorophore aggregation and therefore fluorescence self-quenching, providing a distinct advantage over doping. In the communication in Chem Comm, Scientists at Washington State University, Pullman,WA have developed photostable and biocompatible polymeric nanoparticles with covalently bound perylene dye possessing over 50 times brighter fluorescence as compared to single-dye molecules such as rhodamine 6G.
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They have successfully prepared polymeric nanoparticles with high fluorescent brightness and excellent photostability by covalently embedding a twisted perylene dye into core–shell type polymeric nanoparticles. The high photostability, quantum yield, and unique steric structure of the perylene monomer, coupled with high monomer concentration and core-shell encapsulation enabled by covalent attachment, are believed to mainly contribute to the remarkable brightness and improved photostability. These facile, ultrabright probes ultimately advance the capability for practical applications in live cell imaging, biosensing and in the development of optoelectronic nanodevices.
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